Journal: Biomolecules

Article Title: Fermented Oyster Extract Promotes Osteoblast Differentiation by Activating the Wnt/β-Catenin Signaling Pathway, Leading to Bone Formation

doi: 10.3390/biom9110711

Figure Lengend Snippet: FO increases mitochondrial activity in preosteoblast MC3T3-E1 cells and decreases total cell number. MC3T3-E1 cells were treated with FO (0–200 µg/mL) or 100 nM DEX for 7 days. ( A ) Mitochondrial activity was measured on day 1 (D1), day 3 (D3), day 5 (D5), and day 7 (D7), and ( B ) cell images were collected by Ezscope i900PH phase contrast microscope (×10). Scale bar shows 100 μm. ( C ) Under the same experimental conditions, cell viability and total cell count were measured using flow cytometric analysis. Significant differences among the groups were determined using the one-way ANOVA followed by Bonferroni correction. All data are presented as mean ± SEM (*** p < 0.001, ** p < 0.01, and * p < 0.05 versus untreated group). FO; fermented extract of C. gigas . DEX; dexamethasone, and UT; untreated control.

Article Snippet: Images of each wells were taken with a phase contrast microscope (Ezscope i900PH, Macrotech; Goyang, Gyeonggi-do, Korea).

Techniques: Activity Assay, Microscopy, Cell Counting